The efficiency of tumor-specific pH-responsive peptide-modified polymeric micelles containing paclitaxel
2012
Zhao, Bing-Xiang | Zhao, Yang | Huang, Yue | Luo, Lin-Min | Song, Ping | Wang, Xin | Chen, Su | Yu, Ke-Fu | Zhang, Xuan | Zhang, Qiang
The acidic pH in tumor tissues could be used for targeting solid tumors. In the present study, we designed a tumor-specific pH-responsive peptide H₇K(R₂)₂, which could respond to the acidic pH in tumor tissues, and prepared H₇K(R₂)₂-modified polymeric micelles containing paclitaxel (PTX-PM-H₇K(R₂)₂) in order to evaluate their potential targeting of tumor cells and tumor endothelial cells and their anti-tumor activity in mice with tumor cells. PTX-PM-H₇K(R₂)₂ was prepared by a thin-film hydration method. The in vitro release of PTX from PTX-PM-H₇K(R₂)₂ was tested. The in vitro targeting characteristics of H₇K(R₂)₂-modified polymeric micelles on HUVEC (human umbilical vein endothelial cells) and MCF-7 (human breast adenocarcinoma cells) were evaluated. The in vivo targeting activity of H₇K(R₂)₂-modified polymeric micelles and the in vivo anti-tumor activity of PTX-PM-H₇K(R₂)₂ were also investigated in MCF-7 tumor-bearing mice. The released PTX from the PTX-PM-H₇K(R₂)₂ was not affected by the pH. The targeting activity of the H₇K(R₂)₂-modified polymeric micelles was demonstrated by in vitro flow cytometry and confocal microscopy as well as in vivo biodistribution. PTX-PM-H₇K(R₂)₂ produced very marked anti-tumor and anti-angiogenic activity in MCF-7 tumor-bearing mice in vivo.
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