Bio-callus synthesis of silver nanoparticles, characterization, and antibacterial activities via Cinnamomum camphora callus culture
2020
Aref, Mohamed S. | Salem, Salem S.
Plant tissue culture is considered as a basic and fundamental component of plant biotechnology and is considered a good approach for green synthesis of nanoparticles (NPs) because it is safe, eco-friendly and clean method. In the current study, callus extract of Cinnamonum camphora was utilized to the synthesis of silver nanoparticles (Ag-NPs) for the first time. Callus culture of C. camphora was propagated in murashige and skoog (MS) medium, and sub-cultured for 3 successive times with 4 week intervals. The propagated callus cultures are incubate at different five light conditions using 1500 Lux., white, green, yellow, red and full dark condition. After that, calli which showed the best callus dry biomass was utilized to synthesis Ag-NPs through formation of callus extract. Green synthesized Ag-NPs was characterized by UV–Vis spectroscopy, TEM, SEM-EDX, DLS, FT-IR, and XRD. Results affirmed the ability of callus extract of C. camphora to fabricate spherical Ag-NPs with average size 5.47–9.48 nm at wavelength 420 nm. Also, the crystallographic structure of nanoparticle are confirmed by XRD analysis. Silver ion was represented by 57.3% of the total weight elements in NPs solutions as showed by EDX analysis. In addition, Ag-NPs exhibited broad spectrum activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa with inhibition zones 19.6 ± 0.8, 19 ± 0.5, 17.1 ± 0.7 and 15.1 ± 0.4 mm respectively.
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