An efficient production of a novel carbohydrate polymer Sphingan WL
2018
Li, Hui | Li, Jing | Zhou, Wanlong | Jiao, Xue | Sun, Yajie | Shen, Yaling | Qian, Jin | Wang, Chi-chʻien | Zhu, Hu
BACKGROUND: The sphingan WL produced by Sphingomonas sp. WG has great application potential in the food, ink and other industries. To discover optimal culture conditions for WL production, a gradual optimization method including one‐factor design, Plackett‐Burman design and response surface methodology was employed. Furthermore, the possible reason for the enhancement in WL production was analysed by detecting the gene expression levels in WL biosynthesis using quantitative real‐time PCR. RESULTS: The results showed that the optimal conditions are as follows: seed age, 15–18 h; incubation time, 72 h; inoculum size, 5%; loading volume, 50 mL of medium in 250‐mL shaking flasks; shaking speed, 175 rpm; initial pH of medium, 7.0; and incubation temperature, 32.5°C. WL production was mainly dependent on the concentration of glucose and yeast extract. The statistically optimized culture medium consisted of 72.40 g L⁻¹ glucose, 3.58 g L⁻¹ yeast extract, 3.00 g L⁻¹ K₂HPO₄, 0.10 g L⁻¹ MgSO₄, and 0.10 g L⁻¹ ZnSO₄. Interestingly, a high WL production of 39.95 g L⁻¹, 2.37‐fold the initial production (16.82 g L⁻¹), was obtained, which was due to the higher expression level of WL‐related enzymes under these optimized conditions. CONCLUSIONS: This study provides useful fundamental information for the development of an efficient process for large‐scale WL production. © 2018 Society of Chemical Industry
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