Construction, screening and evaluation of a derivative recombinant adenovirus for the optimal siRNA targeting of a novel gene (HA117)

Zheng, Gaihuan; Guo, Yuxia; Jin, Xianqing; Xu, Youhua; Luo, Qing; Su, Yuxi

Construction, screening and evaluation of a derivative recombinant adenovirus for the optimal siRNA targeting of a novel gene (HA117)

2011

At this study, we screened for an optimal siRNA to target a novel gene named HA117 and constructed and evaluated a recombinant adenovirus carrying the DNA template for the transcription of the optimal HA117 siRNA to examine the function of HA117 and its possible mechanism of action in CW-2 cell lines. An HAi5-carrying recombinant adenovirus (Ad-HAi5) was successfully constructed and evaluated. The results show that, among five pairs of DNA templates, siRNA transcribed from HAi5 gave the strongest interference with the novel gene HA117. The expression of the exogenous HA117 gene increased drug resistance in CW-2 cells. We hypothesized that this action may be a result of HA117 through inhibiting apoptosis in CW-2 cells.

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AGROVOC Keywords

adenoviridae apoptosis dna drug resistance drug resistance genes genetics humans mechanism of action recombination rna screening

Bibliographic information

Published in

Gene

Volume 479 Issue 1 Pagination 10 - 15 ISSN 0378-1119

Publisher

Elsevier B.V.

Other Subjects

Genetic; Rna interference; Small interfering rna; Gene targeting; Small interfering; Templates; Genetic; Multiple; Tumor; Cell line; Hl-60 cells; Transcription (genetics)

Language

English

Type

Journal Article; Text

In AGRIS since: 2024-02-29

Format: MODS

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Links

DOI DOI http://dx.doi.org/10.1016/j.gene.2011.01.018

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