Palladium--a new inhibitor of cellulase activities

Shultz, M.D.; Lassig, J.P.; Gooch, M.G.; Evans, B.R.; Woodward, J.

Palladium--a new inhibitor of cellulase activities

1995

Shultz, M.D. | Lassig, J.P. | Gooch, M.G. | Evans, B.R. | Woodward, J.

Palladium complexes have been shown to strongly inhibit cellobiohydrolase I (CBH I) and endoglucanase II (EG II), two cellulases produced by Trichoderma reesei. Also inhibited were total cellulase (Avicelase) and beta-glucosidase (cellobiase) activities. The catalytic domain of CBH II, the second most abundant component of this cellulase, appeared less susceptible to inhibition by palladium. The inhibition was irreversible and could be prevented if histidine, cysteine or cystine was added to the enzyme reaction mixture simultaneously with the inhibitor. The binding of CBH I to microcrystalline cellulose (Avicel) was unaffected by palladium.

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AGROVOC Keywords

beta-glucosidase beta-glucosidase binding sites cellulase chromatography enzyme activity enzyme inhibitors enzymology glycosidases inhibition kinetics mechanism of action palladium palladium pharmacology trichoderma trichoderma reesei

Bibliographic information

Published in

Biochemical and biophysical research communications

Volume 209 Issue 3 Pagination 1046 - 1052 ISSN 0006-291X

Other Subjects

Cellulases; Cellobiohydrolase i; Endoglucanase ii; O-glycoside hydrolases; Antagonists & inhibitors; Isolation & purification; Endoglucanase i; Cellobiohydrolase ii; Cellulose 1; 4-beta-cellobiosidase; Hypocrea jecorina; Glycoside hydrolases; High pressure liquid

Language

English

Note

2019-12-05

Type

Journal Article; Text

In AGRIS since: 2024-02-29

Format: MODS

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