Overproduction and Substrate Specificity of 3-Isopropylmalate Dehydrogenase from Thiobacillus ferrooxidans
1998
MATSUNAMI, Hideyuki | KAWAGUCHI, Hiroshi | INAGAKI, Kenji | EGUCHI, Tadashi | KAKINUMA, Katsumi | TANAKA, Hidehiko
We constructed an overexpression system in Escherichia coli of the leuB gene coding for 3-isopropylmalate dehydrogenase in Thiobacillus ferrooxidans. E. coli harboring the plasmid we constructed, pKK leuB1, produced 17-fold the enzyme protein of the expression system previously used for purification. The substrate specificity of the enzyme was analyzed with synthetic (2R, 3S)-3-alkylmalates. The 3-isopropylmalate dehydrogenase of Thiobacillus ferrooxidans had broad specificity toward the alkylmalates.
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