Separation of Cytidine 5'-triphosphate Biosynthesized from Cytidine 5'-monophosphate on Ion-exchange Resin and HPLC Analysis of Cytidine Compounds
2008
Shi, L.E. | Ying, G.Q. | Tang, Z.X. | Yi, Y. | Shan, J.F. | Liu, H.Z.
Conditions were studied in the biosynthesis of cytidine 5'-triphosphate (CTP) from cytidine 5'-monophosphate (CMP). A 201 x 7 anion ion-exchange resin was applied for the separation of CTP from CMP. Adsorption isotherm and elution conditions (eluant, eluant concentration, flow rate, sample volume loaded) were investigated. At the same time, a new high-performance liquid chromatography on an anion ion-exchange column WAX-1 with UV detector at 260 nm was developed to measure CMP, cytidine 5'-diphosphate (CDP), and CTP. The retention time for CMP, CDP, and CTP are 0.723, 1.448, and 4.432 min, respectively. This new rapid high-performance liquid chromatography (HPLC) method for the analysis of cytidine compounds in biological sample has a wide linear range with high precision and repeatability.
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