Isolation and Characterization of the Enterobacter cloacae cheR Mutant Defective in Phosphate Taxis
2001
KATO, Junichi | NAGATA, Chinatsu | Yang, Li | KURODA, Akio | IKEDA, Tsukasa | TAKIGUCHI, Noboru | OHTAKE, Hisao
A chemotaxis-defective mutant of Enterobacter cloacae IFO3320, designated EC1, was isolated after N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis. Computer-assisted capillary assays showed that EC1 failed to show chemotactic responses to peptone and inorganic phosphate (Pᵢ). Cloning and sequence analysis showed that EC1 is a cheR mutant, suggesting that Pᵢ taxis by E. cloacae is dependent on a methyl-accepting chemotaxis protein(s)(MCP). EC1 was further mutagenized with NTG to construct cheR pstS and cheR pstA double mutants. A recombinant plasmid pECT01.2, which contained the E. cloacae cheR gene, restored the ability of these double mutants to show chemotaxis toward peptone but not Pᵢ. These results suggest that the phosphate-specific transport (Pst) system, together with a MCP(s), is required for detecting Pᵢ in E. cloacae.
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