An α-Glucuronidase Enzyme Activity Assay Adaptable for Solid Phase Screening
2009
Lee, Charles C. | Wagschal, Kurt | Kibblewhite-Accinelli, Rena E. | Orts, William J. | Robertson, George H. | Wong, Dominic W. S.
Glucuronic acid is a common chemical moiety that decorates the xylan polymer of hemicellulose. This chemical substituent impairs both enzymatic and acidic hydrolysis of xylosidic bonds. The α-glucuronidase enzyme hydrolyzes the 1,2-linked glucuronic acid from the terminal, non-reducing xylose of xylo-oligosaccharides. There are relatively few α-glucuronidase genes in the public databases. We have developed an assay with commercially available reagents that can be used to search DNA libraries for α-glucuronidase genes in a high-throughput, solid phase activity screen.
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