Azulene-Derived Fluorescent Probe for Bioimaging: Detection of Reactive Oxygen and Nitrogen Species by Two-Photon Microscopy
2019
Murfin, Lloyd C. | Weber, Maria | Park, Sang Jun | Kim, Won Tae | Lopez-Alled, Carlos M. | McMullin, Claire L. | Pradaux-Caggiano, Fabienne | Lyall, Catherine L. | Kociok-Köhn, Gabriele | Wenk, Jannis | Bull, Steven D. | Yoon, Juyoung | Kim, Hwan Myung | James, Tony D. | Lewis, Simon E.
Two-photon fluorescence microscopy has become an indispensable technique for cellular imaging. Whereas most two-photon fluorescent probes rely on well-known fluorophores, here we report a new fluorophore for bioimaging, namely azulene. A chemodosimeter, comprising a boronate ester receptor motif conjugated to an appropriately substituted azulene, is shown to be an effective two-photon fluorescent probe for reactive oxygen species, showing good cell penetration, high selectivity for peroxynitrite, no cytotoxicity, and excellent photostability.
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