Deletion of the L7L-L11L Genes Attenuates ASFV and Induces Protection against Homologous Challenge
2021
Zhang, Jingyuan | Zhang, Yanyan | Chen, Teng | Yang, Jinjin | Yue, Huixian | Wang, Lidong | Zhou, Xintao | Qi, Yu | Han, Xun | Ke, Junnan | Wang, Shuchao | Yang, Jinmei | Miao, Faming | Zhang, Shoufeng | Zhang, Fei | Wang, Ying | Li, Min | Hu, Rongliang
African swine fever (ASF), caused by the African swine fever virus (ASFV), is a major epidemic disease endangering the swine industry. Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteristics of ASFV and the possibility of a gene-deleted isolate as a vaccine candidate, the strain SY18ΔL7-11, with deletions of L7L–L11L genes from ASFV SY18, was constructed, and its biological properties were analyzed. The results show that deletion of genes L7L-L11L did not affect replication of the virus in vitro. Virulence of SY18△L7-11 was significantly reduced, as 11 of the 12 pigs survived for 28 days after intramuscular inoculation with a low dose (10³ TCID₅₀) or a high dose (10⁶ TCID₅₀) of SY18ΔL7-11. All 11 surviving pigs were completely protected against challenge with the parental ASFV SY18 on 28 days postinoculation (dpi). Transient fever and/or irregularly low levels of genomic DNA in the blood were monitored in some pigs after inoculation. No ASF clinical signs or viremia were monitored after challenge. Antibodies to ASFV were induced in all pigs from 14 to 21 days postinoculation. IFN-γ was detected in most of the inoculated pigs, which is usually inhibited in ASFV-infected pigs. Overall, the results demonstrate that SY18ΔL7-11 is a candidate for further constructing safer vaccine(s), with better joint deletions of other gene(s) related to virulence.
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