Combined Application of Macroporous Resins and Preparative High-performance Liquid Chromatography for the Separation of Steroidal Saponins from Stems and Leaves of Paris polyphylla
2021
Wang, Feifei | Ma, Xiao | Qu, Liping
In this study, the combined techniques of macroporous resin column chromatography and preparative high-performance liquid chromatography were applied for the separation of steroidal saponins from the stems and leaves of Paris polyphylla. Eight types of macroporous resins were examined using adsorption and desorption tests. Resin HPD-600 showed the maximum effectiveness and thus was selected for the first cleaning-up, in which 30% ethanol was used to remove the undesired constituents and 90% ethanol was used to elute the targets. Paris saponin II (36.2 mg) and dioscin (14.0 mg) were obtained from 500.5 mg of the extracts after purification by preparative high-performance liquid chromatography, with purities above 95% and yields of 81.87 and 72.89%, respectively. These results suggest that combined strategy is a useful and economic method to enrich and purify steroidal saponins from the stems and leaves of P. polyphylla.
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