Soluble, folded and active subtilisin in a protic ionic liquid
2010
Falcioni, Francesco | Housden, Hazel R. | Ling, Zhenlian | Shimizu, Seishi | Walker, Adam J. | Bruce, Neil C.
The activity of proteases chymotrypsin and subtilisin dissolved in a range of protic hydroxylalkylammonium ionic liquids was tested against the model substrate APEE (N-acetyl-l-phenylalanine ethyl ester); activity was only observed for subtilisin in diethanolammonium chloride (DEA Cl), while chymotrypsin was not active in any PIL tested.
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