Artificial seawater for testing the toxicity of pollutants to marine fish, 1: Examination of the suitability by estimating the LC50 and accumulation of Cd for girella Girella punctata Gray
1989
Kuroshima, R. (National Research Inst. of Fisheries Science, tokyo (Japan)) | Kimura, S.
The toxicity and accumulation of Cd for girella, Girella punctata Gray, was examined with the natural seawater and two types of artificial seawater to which EDTA was added (+EDTA) and not added (-EDTA). LC50 values of Cd for girella in the artificial seawater (-EDTA) were practically equivalent to those in the natural seawater, i.e. 34-h, 48-h, 96-h LC50 were 42, 31, 27.5 ppm in the natural seawater and 42, 32.5, 38.5 ppm in the artificial seawater (-EDTA). There was also no difference in Cd concentrations in whole bodies of fish exposed for 96h to 10 ppm or 24 ppm Cd between these two groups. However, in the artificial seawater (+EDTA) 24-h, 48-h. LC50 were 41.8, 29.5 ppm respectively, and 96-h LC50 was estimated less than 24 ppm, besides Cd concentrations in fish exposed to 24 ppm was the highest among the three groups. On the other hand, it was found that the test fish in the artificial seawater (+EDTA) had the least condition factor and contended the highest moisture, which implied that they were different in a nutritive condition from others. Therefore, it was suggested that the discrepancy in LC50 was caused by not the components of the artificial seawater, but the physiological conditions of test fish. In conclusion, the artificial seawater used in this experiment can be applied as test water for marine fish
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