The levels of biotin and biotinidase in bovine milk
1991
Hirano, M. (Tokyo Univ. of Agriculture (Japan)) | Honma, K. | Daimatsu, T. | Hayakawa, K. | Oizumi, J. | Zaima, K. | Kanke, Y.
The concentrations of total biotin and free-form biotin in bovine milk have been determined by a new disc plate method. Increases in the contents of both total and free-form biotins were found in mature milk compared with colostrum. The ratio of free-form biotin to total biotin was approximately 0.81 in colostrum, and it did not change throughout lactation period studied. The activity of biotinidase in bovine milk increased during lactation as the free-form and total biotin contents gradually increased. These observations might suggest that biotinidase plays a role in keeping the ratio of free-form biotin to total biotin constant in bovine milk
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