Single cell cultures of asparagus (Asparagus officinalis L.) and plant regeneration through somatic embryogenesis
1990
Uragami, A. (Hokkaido National Agricultural Experiment Station, Sapporo (Japan))
Single cells of asparagus (Asparagus officinalis L.) were mechanically isolated from cladodes of a fully grown plant and cultured for 4 weeks in a modified Murashige and Skoog (MS liquid medium containing 0.000005M naphthaleneacetic acid (NAA) and 0.000000M 6-benzyladenine (BA). The division of the cells was promoted at high sucrose or glucose concentrations. Transfer of the cells to MS medium without phytohormones induced the formation of a small number of somatic embryos after 2 to 4 weeks. Embryos developed into complete plantlets on solidified MS medium without phytohormones. The somatic embryos cultured on MS medium containing 5 x 0.000000M 2,4-D further dedifferentiated into embryogenic callus. The embryogenic callus was maintained on the same medium and developed complete plantlets through embryos when transferred to MS medium without phytohormones. All 30 plants regenerated via somatic embryos were normal diploids with 20 chromosomes
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