A novel approach for the identification of lipid molecular species: Application of high performance liquid chromatography on fish muscle lecithin molecular species analysis
1985
Takahashi, K. (Hokkaido Univ., Hakodate (Japan). Faculty of Fisheries)
Superiority of the reversed-phase type HPLC was emphasized. In chapter I, it was suggested that dephosphorylation followed by acetylation is effective in separating the critical pairs of the lecithin molecular species on HPLC. The positional isomers were also separated to some extent unlike the triglycerides. And a new matrix model was proposed for the prediction of molecular species of lecithin. In chapter II, the matrix model proposed in chapter I was developed into the rules for molecular species of triglycerides. This idea was based on the expansion of matrix of (2,2) type model for the lecithin molecular species to the matrix of (3,2) type model for the triglyceride molecular species. In chapter III, the computer software for the identification of lecithin molecular species was designated. In chapter IV, from the view point of muscle lecithin molecular species, sardine, mackerel, big-eyed tuna, brown sole, sand flounder, rock fish, Alaska pollack, chum salmon, blue shark, mackerel shark, carp and rainbow trout were examined on HPLC
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