Characterization of an anion transporter in the plasma membrane of barley [Hordeum vulgare] roots
1996
Yamashita, K. (Okayama Univ., Kurashiki (Japan). Research Inst. for Bioresources) | Yamamoto, Y. | Matsumoto, H.
To examine the relationship between H(+)-ATPase and the transport of anions, we investigated the effects of various inhibitors on the activity of the H(+)-ATPase, the transport of protons, and the transport of Cl(-) ions using plasma membrane vesicles prepared from barley roots. Some inhibitors, namely, 4,4-diisothiocyano-2,2-stilbene disulfonate (DIDS) and Zn(2+) ions markedly inhibited H(+)-ATPase activity. Other compounds, such as phenylglyoxal (PGO) and niflumic acid (NIF), inhibited H(+)-ATPase activity by 20-30%, while anthracene-9-carboxylate (A-9-C) and tetraethylammonium chloride (TEA-Cl) had little effect on this activity. The ATP-dependent acidification of the interior of vesicles was strongly dependent on the presence of permeant anions, such as chloride (Cl(-)) and nitrate (NO3(-)), and it was completely inhibited by 0.2 mM Nif
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