High-rate seawater denitrification utilizing a macro-porous cellulose carrier
1997
Catalan, Ma.A.S. (Tsukuba Univ., Ibaraki (Japan). Inst. of Applied Biochemistry) | Wang, P.C. | Matsumura, M.
Continuous denitrification experiments were undertaken using marine denitrifying bacteria immobilized in a macro-porous cellulose carrier. The denitrifying capacity of this immobilized system was examined using an ejector-type bioreactor at pH 8.0, 30 degrees C, under anaerobic conditions. In addition, the immobilized denitrifying bacteria were isolated and characterized. The results showed that complete denitrification will not occur unless (i) a phosphorus (g) per NO3-N (g) ratio (P/NO3-N) of 0.029, (ii) 0.01% trace element solution at 280 g/cubic-meter NO3-N concentration and (iii) a methanol concentration at 30% more than the the oretical carbon concentration requirement are maintained. Copper enhanced nitrite reduction at an influence Cu (g) per influent NO3-N (g) ratio (Cu/NO3-N) of 0.002. Based on these results, a feeding medium was formulated which not only reduced costs but, more importantly, allowed the maximum NO3-N loading rate of 24.8 kg-N/cubic-meter-carrier/d. This value is comparable to that of freshwater in which a macro-porous cellulose carrier was also employed, indicating the insensitivity of the immobilized denitrifying bacteria to salinity. The morphological and physiological characteristics of the immobilized denitrifying bacteria showed similarities to Hyphomicrobium spp
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