Propagation of Drosera spp. from leaf explant in vitro
2001
Yamamoto, T. (Minamikyushu Univ., Takanabe, Miyazaki (Japan). Faculty of Horticulture) | Nakagawa, N.
Leaf explants taken form the donor plant derived from aseptic seeding of Drosera spp (Drosera "8" South Africa) were cultured on the half strength Murashige & Skoog media supplemented with various concentrations of benzyladenine (BA) and naphthaleneacetic acid (NAA) . The hormne-free medium gave the best results for the formation of adventitious shoots from the leaf explant and plant regeneration. In the earliset case, red protuberances were formed on the distal part of leaf explant after 14 days of culture on the hormone-free medium. The red protuberances developed into adventitious shoots, resulting in the formation of rosette of drosera spp. Benzyladenine in the medium suppressed the rooting of adventitious shoots in vitro. After acclimatization, the rosettes regenerated were grown in a greenhouse. In two-three months after acclimatization, flowering was observed on the top of elongated flower stalks of the regenerated Drosera spp.
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