Enzymatic synthesis of novel oligosaccharides from L-sorbose, maltose, and sucrose using kojibiose phosphorylase
2001
Chaen, H. (Hayashibara Biochemical Labs. Inc., Okayama (Japan)) | Nishimoto, T. | Nakada, T. | Fukuda, S. | Kurimoto, M. | Tsujisaka, Y.
Glucosyl-L-sorbose, -maltose, and -sucrose were synthesized using kojibiose phosphorylase (KPase) from Thermoanaerobacter brockii ATCC35047 with beta - D-glucose-1-phosphate (beta - G1P) as a glucosyl donor. One disaccharide and two trisaccharides thus synthesized were isolated by Toyopearl HW-40S column chromatography. The results of KPase digestion, methylation analysis, and 13C-NMR studies indicated that these oligosaccharides were alpha - D-glucopyranosyl-(1 -> 5)- alpha - L-sorbopyranose, alpha - D-glucopyranosyl-(1 -> 2)- alpha - D-glucopyranosyl-(1 -> 4)-D-glucopyranose (4- alpha - D-kojibiosyl-glucose), and alpha - D-glucopyranosyl-(l -> 2)- alpha -D-glucopyranosyl-(l -> 2)-P-D-fructofurano-side, which are all novel oligosaccharides. Glucosyl-L-sorbose was partially hydrolyzed to glucose and L-sorbose by alpha - glucosidases, while glucosyl-sucrose and glucosyl-maltose were not hydrolyzed by glucoamylase, alpha-glucosidases, nor CGTase.
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