Detection and identification of Oncorhynchus masou virus (OMV) by polymerase chain reaction (PCR)
2001
Aso, Y. (Hokkaido Univ., Sapporo (Japan)) | Wani, J. | Klenner, D.A.S. | Yoshimizu, M.
A polymerase chain reaction (PCR) was established for detection and identification of Oncorhynchus masou virus (OMV). A set of primers was prepared for amplification of a target gene of DNA from OMV reference strain 00-7812, and the PCR was able to amplify the DNA of a 439bp from four representative strains of OMV isolated from masu salmon (Oncorhynchus masou), coho salmon (O.kisutch) and rainbow trout (0.mykiss), and from liver, kidney, brain and nervous tissues of OMV infected fish. It was also able to amplify about 800bp from Herpesvirus salmonis, the reference strain of salmonid herpesvirus type 1, isolated from rainbow trout in the USA.
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