A simple method for enrichment of uninucleate conidia of Aspergillus oryzae
2002
Hara, S. (Noda Inst. for Scientific Research, Chiba (Japan)) | Tsuji, R.F. | Hatamoto, O. | Masuda, T.
Aspergillus oryzae produces multinucleate conidia, which makes the obtaining of homokaryons labor-intensive. Analysis of conidia by flow cytometry clarified the relationship that conidia of lower nuclear number were smaller in size. Based on this, we have developed a simple way to enrich uninucleate conidia with a membrane filter. Our results also suggest that the method is useful for elimination of heterokaryons.
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