Analysis of positional isomers of alpha-linked glucooligosaccharides by p-aminobenzoic acid ethyl ester-conversion method
2002
"Kobayashi, I. (Honen Corp., Tokyo (Japan)) | Yasuno, S. | Hashimoto, H. | Ikura, K. | Nakano, H. | Kitahata, S. "
"A new method for simultaneously analyzing positional isomers of alpha-linked glucooligosaccharides was described. Four alpha-glucobioses (kojibiose, nigerose, maltose and isomaltose) were analyzed by using the r-aminobenzoic acid ethyl ester (ABEE)-conversion method under the following conditions: mobile phase, 0.1 M ammonium acetate buffer (pH 4.0) containing 12% acetonitrile; column temperature, 25degC. Furthermore a mixture of four alpha-glucobioses, five alpha-glucotrioses, 2E2-O-alpha -D-glucosyl-D-maltose (kojibiosyl glucose), 3E2-O-alpha -D-glucosyl-D-maltose (nigerosyl glucose), 4-O-alpha -D-glucosyl-D-maltose (maltotriose), 6E2-O-alpha-D-glucosyl-D-maltose (panose), 6-O-alpha-D-glucosyl-D-maltose (isomaltotriose) and glucose was able to be analyzed by using the ABEE-conversion method with 0.1 M ammonium acetate buffer (pH 4.0) containing 10.5% acetonitrile as an eluent and a column temperature of 25degC. This separation was further improved by connecting two columns in series. The relationship between the peak areas and quantities of ABEE-converted alpha-linked glucooligosaccharides was linear in the range of 30 pmol to 3 micromol."
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