Effects of serum deprivation on myofibrillar proteolysis in chick myotube cultures
2003
Nakashima, K. (National Inst. of Livestock and Grassland Science, Tsukuba, Ibaraki (Japan)) | Nonaka, I. | Masaki, S.
We examinedthe effects of serum deprivation on myofibrillar proteolysis in chick myotubes. Myotubes were incubated with serum-free medium for 24 hours. N sup t-methylhistidine release, as an index of myofibrillar proteolysis, as well as protease activities such as calpain, proteasome, and cathepsins (B + L and D) activities were increased by serum deprivation. These results indicate that serum deprivation induces calpain, proteaosme, and cathepsins activities, resulting in an increase in myofibrillar proteolysis in chick myotubes.
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