Improved efficiency in differentiating races of rice blast fungus [Magnaporthe grisea] using a multi-cell tray and plant growth regulator
2005
Ashizawa, T. (National Agricultural Research Center for Tohoku Region, Morioka (Japan)) | Zenbayashi, K.S.
The method of Goto and Yamanaka (1968) was modified to improve efficiency when differentiating of Pyricularia grisea races. Three seeds of one of each of 12 Japanese differential rice varieties were sown in a 3-cm square cell of a 12-cell tray (3x4 cell) and treated immediately with 10ml of 2.5 micro g/ml uniconazole (a plant growth regulator) to yield dwarf seedlings. The seedlings (5-leaf stage) were then inoculated with one of 23 blast isolates (6 races), and covered with plastic cases to prevent contamination from the other isolates. The blast races identified by the method were consistent with those identified with the method of Goto and Yamanaka. With the use of dwarf seedlings and protection cases, many blast isolates can be identified at the same time.
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