Improvement of a PCR detection method for Perkinsus olseni isolated from Manila clam, Ruditapes philippinarum
2007
Nakatsugawa, T.(Kyoto-fu. Inst. of Oceanic and Fishery Science, Miyazu (Japan))
The agent of perkinsosis on the clam Ruditapes philippinarum in Japan was identified as Perkinsus olseni (Protozoa Apicomplexa) on sequencing of the ITS region. The author detected Perkinsus sp. on the clam by P. olseni and P. marinus-specific polymerase chain reaction (PCR) and a fluid thioglycollate medium technique (FTM). It was considered that PCR was less effective than FTM in the investigation of Perkinsus sp. infection. The author compared two PCR methods. An ordinary PCR reaction mixture was used in the first PCR method. The second PCR (ADP PCR) was conducted using a PCR reaction mixture to which Ampdirect Plus (Shimadzu) was added. ADP PCR showed a better detection sensitivity for Perkinsus sp. than ordinary PCR and was equal to FTM.
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