A study on the mass propagation of skunk cabbages (Symplocarpus foetidus)
2008
Kitamura, H.(Shiga-ken. Agricultural Technology Promotion Center, Azuchi (Japan)) | Mori, M. | Nishibori, Y. | Ohtani, H.
Various methods of mass-propagating skunk cabbages were investigated. First, a method based on tissue culture was investigated. Multiple shoots were formed by culturing shoot apexes on a basal medium (MS) supplemented with 0.1 mg/l naphthalene acetic acid (NAA) and 1-5 mg/l benzyladenine (BA). Each shoot was divided and transplanted to fresh medium of the same composition. These processes were repeated to further propagate multiple shoots. Culture temperature was optimized at 20 deg C. Well grown multiple shoots were readily rooted in an MS medium supplemented with 30 g/l sucrose and 3.5 g/l Gelrite, resulting in a large number of young seedlings. Next, a method of efficiently producing seedlings from seeds was investigated. Seeds were collected in late-July and exposed to low temperature (10 deg C, 3 months), after which they were sown in bog moss and kept under shade. More than 37% of the seeds germinated the following year (2nd year), and more than 90% germinated in the 3rd year. Furthermore, a method based on aseptic sowing was investigated. Seeds were collected as described above, and exposed to low temperature (10 deg C, 30 days), after which they were aseptically sown in an MS medium supplemented with 0.1 mg/l BA and 0.5 mg/l NAA, or a hormone-free MS medium. About four months later, more than 40% of the seeds had germinated, irrespective of the addition of plant hormones. Hence, this method was shown to shorten the seedling-raising period.
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