Occurrence and molecular characterization of Kyuri green mottle mosaic virus isolated from oriental melon in Korea
2009
Kim, O.K.(Tokyo Univ. of Agriculture, Atsugi, Kanagawa (Japan)) | Lee, K.W. | Natsuaki, K.T.
Mosaic diseases were detected in oriental melons cultivated in Seongju County, Kyungpook Province, Korea. The oriental melons were infected by Cucumber green mottle mosaic virus (CGMMV), Watermelon mosaic virus 2 (WMV2) and some viruses in the genus Potyvirus, according to ELISA detection. Additionally, from one melon, a virus was isolated and identified as Kyuri green mottle mosaic virus (KGMMV) by electron microscopy, biological analysis, RT-PCR and sequencing of the movement protein (MP) and coat protein (CP) genes. Results of host reactions of the KGMMV isolate (KGMMV-KOM) were similar to that described for KGMMV-C1 isolated from Japan, while KGMMV-KOM showed latent infection on oriental melon. Although the CP gene of KGMMV-KOM comprised 486 nts, the same as previously reported KGMMV, the MP gene comprised 786 nts, which is shorter than that of other KGMMV (789 nts). Phylogenetic analysis based on the nucleotide sequences of MP and CP genes was also conducted and KGMMV-KOM was found to belong to the major cluster of KGMMV, showing molecularly conservative nature of this species. This is the first report of KGMMV infecting on oriental melon in Korea.
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