Isolation of Lactococcus garvieae showing no agglutination with anti-KGsup(-) phenotype rabbit serum
2015
Oinaka, D. (University of Miyazaki, Miyazaki (Japan). Faculty of Agriculture) | Yoshimura, N. | Fukuda, Y. | Yamashita, A. | Urasaki, S. | Wada, Y. | Yoshida, T.
Lactococcus garvieae-like bacteria, which did not agglutinate with a rabbit antiserum against L. garvieae capsulated cells (KGsup(-) phenotype cells), were isolated from diseased yellowtail Seriola quinqueradiata in 2012. The non-agglutinating L. garvieae-like bacteria reacted positively in a species-specific PCR targeting the 16S rRNA gene of L. garvieae. DNA-DNA relatedness values between reference L. garvieae strains and the non-agglutinating strains were higher than 70%. Almost complete sequencing of the 16S rRNA gene of the non-agglutinating strains revealed high similarity with that of L. garvieae ATCC49156. The non-agglutinating strains showed the same morphological and biochemical characteristics as reference L. garvieae strains. These results confirm that the non-agglutinating strains are L. garvieae. However, the present strains differed from the reference strains in terms of the lytic bacteriophage susceptibility. Serological analysis revealed that autoclave-extracted cellular antigens of the non-agglutinating strains did not react to antiserum raised against L. garvieae KGsup(-) phenotype cells. Although the non-agglutinating strains were isolated from different prefectures, they showed similar patterns in biased sinusoidal field gel electrophoresis (BSFGE).
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Agriculture, Forestry and Fisheries Research Information Technology Center