Molecular detection of potato yellow vein virus (PYVV) in leaves and shoot-tubers of Solanum phureja from Colombia
2009
Guzmán, M., Laboratorio de Virus Vegetales. IBUN, Universidad Nacional de Colombia, Bogotá | Franco, L., Facultad de Ciencias, UMNG, Bogotá, Colombia | Rodríguez, P., Laboratorio de Virus Vegetales. IBUN, Universidad Nacional de Colombia, Bogotá
Spanish; Castilian. PYVV, family Closteroviridae, genus Crinivirus, is a phloem limited virus with a tripartite, ssRNA genome, covered with a two protein capsid, found in Andean countries. Symptoms include yellowing of the leaf apex until leaflets are fully cholorotic. PYVV is vegetatively transmitted and its vector is Trialeurodes vaporariorum. In this work, PYVV was detected by RT-PCR (retrotranscriptase PCR) in leaves and shoot-tubers from symptomatic Solanum phureja (egg yolk) plants. ds RNA was used as template for RT-PCR, using primers for part of the major CP gene. A band of 296 pb was observed in leaves and tuber-shoots samples from 20 symptomatic plants, but not from virus free controls. SSCP technique (single strand conformational polymorphism) from RT-PCR products was implemented in polyacrilimide gels obtaining at least two different patterns that suggest variability. PYVV was also detected by in situ hybridization of the phloem on petioles and tuber-shoots prints on membranes treated with a DIG-labelled probe (296pb) and revealed with alkaline phosphatase. Potato crops in Colombia are important since 161 000 ha of S. tubersosum and S. phureja are grown per year. All the grown varieties are susceptible to PYVV, its incidence is high and the yield reduction is important. Although this virus is considered as a quarantine pathogen, there are no available antibodies for PYVV detection. The relevance of this work is to provide a molecular detection method for PYVV in shoot-tubers as well as leaves useful for certification, confirmation of its localization in the phloem and detection of possible variability. Grants: Proyect 2007S4654-69 MADR/UNAL/UMNG.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Instituto de Investigaciones de Sanidad Vegetal