Arabidopsis Synaptotagmin 1 is required for the maintenance of plasma membrane integrity and cell viability
2008
López-Cobollo, Rosa María | Salinas, Julio | Valpuesta, Victoriano | Botella, Miguel Angel
15 páginas, 6 figuras.-- et al.
Show more [+] Less [-]Plasma membrane repair in animal cells uses synaptotagmin 7, a Ca2+-activated membrane fusion protein that mediates delivery of intracellular membranes to wound sites by a mechanism resembling neuronal Ca2+-regulated exocytosis. Here, we show that loss of function of the homologous Arabidopsis thaliana Synaptotagmin 1 protein (SYT1) reduces the viability of cells as a consequence of a decrease in the integrity of the plasma membrane. This reduced integrity is enhanced in the syt1-2 null mutant in conditions of osmotic stress likely caused by a defective plasma membrane repair. Consistent with a role in plasma membrane repair, SYT1 is ubiquitously expressed, is located at the plasma membrane, and shares all domains characteristic of animal synaptotagmins (i.e., an N terminus-transmembrane domain and a cytoplasmic region containing two C2 domains with phospholipid binding activities). Our analyses support that membrane trafficking mediated by SYT1 is important for plasma membrane integrity and plant fitness
Show more [+] Less [-]A.L.S. was supported by a FPU fellowship from the Ministerio de Educación y Ciencia and F.B. by SAV Grant APVV-0432-06. This work was supported by funding from El Ministerio de Educación y Ciencia (Grants BIO2005-4733 and BIO2008-1709), grants from the Bundesministerium für Wirtschaft und Technologie via Deutsches Zentrum für Luft und Raumfahrt (Cologne, Germany; Project 50WB 0434), from the European Space Agency (ESA-ESTEC Noordwijk, The Netherlands; MAP Project AO-99-098), and from the Ente Cassa di Risparmio di Firenze (Italy)
Show more [+] Less [-]Peer reviewed
Show more [+] Less [-]Bibliographic information
This bibliographic record has been provided by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria