Human calcitonin tetrameric gene : comparative expression in yeast and transgenic potato plants
1999
Mironova, R. | Ivanov, I. (Institute of Molecular Biology, Sofia (Bulgaria)) | Ofoghi, H. | Moazami, N. | Domonsky, N.
Human calcitonin is a 32 amino acid peptide participating in the regulation of calcium and phosphorus metabolism in humans.It is used in clinics for treatment of diseases related to bone decalcification.A synthetic hCT tetrameric (hCT1) gene is now cloned in binary vectors for gene expression in yeast and plants.The plasmid for expression in plants carries the 35S cauliflower mosaic virus promoter linked to the tobacco etch virus 5'-nontranslated leader sequence acting as a translation enhancer.Two strains of yeast cells and one cultivar of potato were transformed and expression of the hCT1 gene.
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