Multiplex PCR assay for identification of Erwinia amylovora - the causative agent of fire blight
2006
Kabadzhova, P. | Atanasova, I. | Moncheva, P.
Seven Bulgarian strains of Erwinia amylovora isolated from chokeberry and strawberry, previously identified by single PCR amplification of a specific region of plasmid pEA29 and genome ams-region were subjected to multiplex PCR using two pairs of primers- pEA29A/pEA29B and AJ245/AJ246. These primers allowed the identification of the strains in one-step PCR simultaneously in two genetic loci. The conditions of the multiplex PCR were avoided. The multiplex PCR proposed is a not time-consuming and reliable method for rapid identification and discrimination of E. amylovora from the closed E. pyrifoliae species.
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