Optimization of laboratory conditions for biosynthesis of type a Trichothecenes
2007
Bocarov-Stancic, A.S.,Hold. Comp. Center for Bio-Ecology, Zrenjanin (Serbia) | Jacevic, V.M.,Military Medical Academy, Belgrade (Serbia). National Poison Control Center | Resanovic, R.D.,Faculty of Veterinary Medicine, Belgrade (Serbia). Institute for Poultry Diseases | Bijelic, M.B.,Hold. Comp. Center for Bio-Ecology, Zrenjanin (Serbia)
Type A trichothecenes, T-2 toxin and diacetoxyscirpenol -DAS, belong to one of the most toxic groups of fusariotoxins. Having in mind the importance of these substances, the aim of this study was to carry out the optimization of laboratory conditions under which screening of Fusarium spp. isolates from Serbia, regarding T-2 toxin and DAS production should be done. Four culture of Fusarium sporotrichioides, originating from different regions throughout the world, were under present investigation: ITM-391 (Italy), KF-38/1 (Poland), M-1-1 (Japan) and R-2301 (Germany). According to the previous literature data, all of these isolates were T-2 toxin producers, and some of them were also DAS producers. The influence of medium composition as well as aeration on biosynthesis process of these mycotoxins, in vitro conditions was investigated. In the case of most Fusarium sporotrichioides isolates, the highest yields of T-2 toxin and DAS were achieved under the conditions of more intense aeration, and with the use of glucose (5 or 20%) as a C atom source. Fermentation in semi-synthetic liquid medium, using a rotary sheaker was more suitable for screening the toxicity of the fungal isolates in pure culture because of: shorter period of incubation, more simpler sample preparation, obtaining less interfering materials in crude toxin extracts, and possibility for more precise definition of factors influencing the yield of trichothecenes.
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