Инфекция гриппа птиц, вызванная вирусом подтипа H7N1

Abramova, L.Yu.; Chvala, I.A.; Babin, Yu.Yu.; Mudrak, N.S.; Borisov, A.V.; Drygin, V.V., Federal Centre for Animal Health, Yurievets; Vlasov, N.A., Federal Veterinary and Phytosanitary Inspection Service, Ufa

Avian influenza infection A/H7N1 | Инфекция гриппа птиц, вызванная вирусом подтипа H7N1

2010

Clinical signs and anatomico-pathological changes under experimental avian influenza (AI) caused by high- and low-pathogenic epizootic strains of H7N1 virus. Avian influenza virus-non-resistant 30-day-old chickens were infected intranasally with low-virulent (LV) strain A/turkey/Italy/3675/99 N7N1 and high virulent (HV) strain A/turkey/Italy/4580/99 H7N1. commercial tests Flu Detect (Syn biotics, France) and AIV Ag (Anigen, Korea) and virus shedding in chicken embryos were used to detect AIN virus (AIV) in samples of cloacal and oropharyngeal smears and pathomaterial. As is shown, AI caused by HV virus A/turkey/Italy/4580/99 H7N1 proceeded in a superacute form with typical clinical signs and pathological changes. The efficiency of the Flu Detect for testing cloacal and oropharyngeal smears was 84.62%and 53.85%, AIV Ag – 61.54% and 38.46%, respectively. During virus shedding in chicken embryos and using test-systems AIV was detected in all samples of pathomaterial taken from dead fowls in 24 and 48 h after infection testifying thereby to generalized infection. IV caused by A/turkey/Italy/3675/99 N7N1 proceeded mainly asymptomatically, with suppression and diarrhea. The Flu Detect revealed AIV in cloacal s,ears not less than in 10 days after infection, in oropharyngeal smears – at day 3. Virus-neutralizing antibodies in chicken blood serum in IHT and DPT were found in 14 days after infection. It is concluded that to detect and identify HV AIV during an outbreak of disease, it is more advisable to use a commercial Flu Detect kit having a high diagnostic efficiency

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