Испытание аденовирусных векторов CELO-H5 и CELO-H7, экспрессирующих гемагглютинин вируса гриппа птиц, на коммерческих цыплятах

Shul'pin, M.I.; Chvala, I.A., Federal Centre for Animal Health, Yurievets

Testing of adenovirus vectors CELO-H5 and CELO-H7 expressing avian influenza virus hemaglugglutinini commercial chicks | Испытание аденовирусных векторов CELO-H5 и CELO-H7, экспрессирующих гемагглютинин вируса гриппа птиц, на коммерческих цыплятах

2010

Shul'pin, M.I. | Chvala, I.A., Federal Centre for Animal Health, Yurievets (Russian Federation)

The immune response in White Leghorn chickens after administration of recombinant adenoviruses CELO (Chicken embryo lenthal orphan virus) carrying a hemagglutinin gene of subtypes H5 and H7 of avian influenza virus (AIV) was studied. It protective ability for challenge of twice immunized chickens with strain of high pathogenic AIV A/duck/Novosibirsk/02/05 (H5N1) and reference strain A/fowl/Rostock/45/1934 (H7N1)was determined. An evaluation of the immune response strength by vaccinating chickens with CELO-H5, CELO-H7 and a mixture of CELO-H5 and CELO-H7 showed the best result when CELO-H5 was used. So, 4 weeks after vaccination 94% of chickens immunized with CELO-H5 has a titer in HIT 1:4. Under a challenge in 7 weeks after the 1-st immunization the fowls vaccinated with CELO-H5 or CELO-H7 alone remained clinically healthy for 10 days (the observation period); under vaccination with the mixture of CELO-H5 and CELO-H7 and infection with A/duck/Novosibirsk/02/05 H5N1 survived 75%, A/fowl/Rostock/45/1934 H7N1 – 87%. Thus, vaccination of chickens with recombinant CELO allowed producing an immune response to hemagglutinin H5 and H7 AIV having protective properties. Simultaneous using CELO-H5 and CELO-H7 reduced the vaccination efficiency preserving protective immunity at the high level

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