In vitro propagation of Lilium martagon L. var. cattaniae Vis. and evaluation of genotoxic potential of its leaves and bulbs extracts | In vitro razmnoževanje in ocena genotoksičnosti izvlečkov listov in čebul taksona Lilium martagon L. var. cattaniae Vis.

English. Lilium martagon L. var. cattaniae Vis. (Liliaceae) is endemic plant of Dinaridi mountain. In this work we established protocol for fast in vitro propagation and multiplication of Lilium martagon var. cattaniae. The aim was to enable fast production of plant material as potential source of pharmaceutically valuable secondary metabolites. Seeds of L.martagon var. cattaniae were germinated on a Murashige and Skoog basal medium with a supplement of 0.15 mg/l gibberellic acid (GA3), and multiplication was performed on MS medium supplemented with 0.1 mg/l gibberellic acid (GA3), 0.2 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l 6-ben- zylaminopurine (BAP). We used ultrasound assisted extraction to prepare extracts of leaves and bulbs of Lilium martagon var. cattaniae, which were evaluated for their genotoxic potential using Allium test and cytokinesis-block micronucleus test in human lymphocytes culture. There was statistically significant difference between all used concentrations of lilium extracts and control on proliferation of cells of root tip of onion (Allium cepa). In cytokinesis-block micronucleus test no statistically significant difference between frequencies of analyzed parameters in samples treated with tested concentrations of extracts and control was obtained.

Slovenian. Lilium martagon L. var. cattaniae Vis. (Liliaceae) je endemični takson Dinaridov. Članek podaja načrt za hitro in vitro propagacijo in multiplikacijo taksona Lilium martagon L. var. cattaniae. Cilj je bil omogočiti hitro propagacijo rastlinskega materiala, ki je potencialni izvor metabolitov, pomembnihza farmacijo. Semena taksona Lilium martagon var. cattaniae smo kalili na Murashige in Skoog (MS) hranljivi podlagi, kateri smo dodali 0,15 mg/l giberelinske kisline (GA3). Multiplikacija je prav tako potekala na MS podlagi, ki smo ji dodali 0,1 mg/l giberelinske kisline (GA3), 0,2 mg/l indol-3-maslene kisline (IBA) in 0,5 mg/l 6-benzilaminopurina (BAP). Za pripravo izvlečkov listov in korenin taksona Lilium martagon var. cattaniae smo uporabili ultrazvočno ekstrakcijo. Za ugotavljanje genotoksičnega potenciala teh izvlečkov je bil uporabljen Allium test in citokinetski blok mikronukleus test na humanih limfocitih. Različne koncentracije izvlečkov lilij so imele značilen vpliv na proliferacijo celic korenin čebule (Allium cepa) glede na kontrolo. Pri citokinetskem blok mikronukleus testu ni bilo značilnih razlik med frekvencami analiziranih parametrov pri vzorcih obravnavanih z različnimi koncentracijami izvlečkov in kontrolo.