Effect of aero-anaerobiosis on decarboxylase activity of selected lactic acid bacteria | Vliv aerobního-anaerobního prostředí na dekarboxylázovou aktivitu vybraných bakterií mléčného kvašení
2010
Buňková, L., Univerzita Tomáše Bati ve Zlíně (Czech Republic) | Buňka, F., Univerzita Tomáše Bati ve Zlíně (Czech Republic) | Pollaková, E., Univerzita Tomáše Bati ve Zlíně (Czech Republic) | Podešvová, T., Univerzita Tomáše Bati ve Zlíně (Czech Republic) | Dráb, V. | Kráčmar, S., Univerzita Tomáše Bati ve Zlíně (Czech Republic)
Biogenic amines are undesirable compounds produced in foods mainly through bacterial decarboxylase activity. The aim of this study was to investigate some environmental conditions (particularly aero/anaerobiosis, sodium chloride concentration (0–2% w/w), and amount of lactose (0–1% w/w)) on the activity of tyrosine decarboxylase enzymes of selected six technological important Lactococcus lactis strains. The levels of parameters tested were chosen according to real situation in fermented dairy products technology (especially cheese-making). Tyramine was determined by the ion-exchange chromatography with post-column ninhydrine derivatization and spectrophotometric detection. Tyrosine decarboxylation occurred during the active growth phase. Under the model conditions used, oxygen availability had influence on tyramine production, anaerobiosis seemed to favour the enzyme activity because all L. lactis strains produced higher tyramine amount.
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