Segregation analysis and comparison of hop (Humulus lupulus L.) microsatellite markers variability
2010
Vašek, J., Czech University of Life Sciences, Prague (Czech Republic) | Vejl, P., Czech University of Life Sciences, Prague (Czech Republic) | Nesvadba, V. | Čílová, D., Czech University of Life Sciences, Prague (Czech Republic) | Zoufalá, J., Czech University of Life Sciences, Prague (Czech Republic) | Sedlák, P., Czech University of Life Sciences, Prague (Czech Republic) | Sedláková, V., Czech University of Life Sciences, Prague (Czech Republic)
Wild hop (Humulus lupulus L., Cannabaceae) is a dioecious perennial plant, native to the northern hemisphere and its cultural forms are mainly used in brewery. Populations of wild hop are a potential source of new features and therefore valuable for breeders. Further studies of hop populations are informative about the hop evolution as a species. Tested was the mode of inheritance, linkage and possibility of null allele presence at 8 microsatellite loci in 118 plants of one full-sib family and their genetic variability was compared with genetic diversity of 57 plants supposed to be natural population in the locality Vinařická hora. We found that one locus does not fit to Mendelian segregation ratio, two are in linkage and in two loci the occurence of null allele is possible. It was also found that 8 genotypes of hybrid progeny do not have expected combination of parental alleles. Heterozygote excess, low number of individual genotypes, small sampling area and population structure assigned highly similar pattern, as a structure of hybrid progeny are indicators that population Vinařická hora.
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