Influence of energy components to culture media on the bovine sperm motility parameters in vitro | Vplyv energetických zložiek kultivačných médií na pohybové parametre bovinných spermií v podmienkach in vitro
2010
Kňažická, Z., Slovenská poľnohospodárska univerzita v Nitre (Slovak Republic). Fakulta biotechnológie a potravinárstva | Tvrdá, E. | Kerti, A. | Massányi, P. | Lukáč, N. | Bulla, J.
The aim of this study was to analyse the influence of different energetic substrates used in culture media, on maximum viability and motility of bovine spermatozoa during a 24-hour cultivation in vitro. We compared undiluted ejaculate (N) with groups to which different culture media (A, B, C, D, E, F, G) were applied during three time periods (time 0, time 1 and time 24) using Sperm VisionTM CASA system. Our experimental study shows that the average motility and progressive motility of bovine spermatozoa in the control sample value decreased during a 24-hour cultivation in comparison with time 0 and time 1. The most significant inhibitory effect on the bovine sperm motility parameters was while applying media E 5.890 plusmin 3.111%. The highest motility value was detected while applying culture media G 96.0 plusmin 2.31%. A significantly high motility (P less then 0.001) was detected between N : A, N : F a N : G. Average progressive motility value decreased in all of the used media. The lowest progressive motility value was detected in group E, containing glucose (5%) and sacharose (5%), 4.615 plus minus 1.182%. The maximum viability of bovine spermatozoa was while applying culture media G 45.11 plusmin 5.395%. This adequate culture medium i.e. glucose (5%), trehalose (1%) and sacharose (5%), ensured sufficient energy supply for their survival in vitro. A significantly high progressive motility (P less then 0.001) was detected in groups E and G when comparing with the control sample. Our results suggest that the optimal culture medium for sperm cultivation should contain glucose, sacharose and trehalose in an adequate proportion, using glucose as the dominant energy substrate.
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