Applications of microsatellite and sequence tagged site marker to detect blast resistance in local rice (Oryza sativa L.)
2006
Nguyen Manh Cuong | Nguyen Thi Lang; | Bui Chi Buu
Blast, caused by Pyricularia grisea Cav., is one of the major fungal diseases infected rice (Oryza sativa L.) in Vietnam. Local varieties have been considered as genetic sources of disease resistance among crops. This study aims at using DNA marker assisted selection (MAS) select varieties that have blast resistance genes such as Pi-2 (t), Pi-5 (t) and Pi-3 (t). STS. marker with RG64 was used to detect 100 local varieties to find gene Pi-2 (t) in chromosome 6 and SSR marker with RM21 to find genes Pi-5 (t) and Pi-3 (t) in chromosome 4. Phenotypic selection was used to compare with genotype to check how accurate the methods were. Polymorphisms in varieties show that MAS reached an accuracy of 100 percent in STS. marker with RG64 and 99.49 percent in SSR marker with RM21. These methods are enough accurate to apply in practice to select varieties that have blast resistance genes for breeding rice. Nang Huong, Lem Bui, Soi Da, Nang Tra, Nang Tra Ran Doc, Soc Nau, and Te Tep having 3 blast resistance genes Pi-2 (t), Pi-5 (t), and Pi-3 (t), are considered as valuable material for pyramiding resistance genes to created durably resistant varieties.
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