A simple and rapid method of sample preparation from culture filtrate of M. tuberculosis for two-dimensional gel electrophoresis
2010
Kumar, Gavish(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology) | Shankar, Hari(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology) | Bisht, Deepa(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry) | Sharma, Prashant(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry) | Singhal, Neelja(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Biochemistry) | Katoch, V.M.(Indian Council of Medical Research Department of Health Research) | Joshi, Beenu(National JALMA Institute for Leprosy and Other Mycobacterial Diseases Department of Immunology)
Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.
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