Screening and characterisation of gamma-aminobutyric acid (GABA) producing lactic acid bacteria isolated from Thai fermented fish (Plaa-som) in Nong Khai and its application in Thai fermented vegetables (Som-pak)
2020
TANAMOOL, Varavut | HONGSACHART, Piyorot | SOEMPHOL, Wichai(Khon Kaen University Faculty of Technology Fermentation Research Center for Value Added Agricultural Products)
Abstract This study aimed to evaluate the capability of lactic acid bacteria (LAB) isolated from different kinds of fermented fish products (Plaa-som) on the production of γ-aminobutyric acid (GABA). Among them, isolate L10-11, identified as Lactobacillus plantarum, offered the highest GABA production and was selected for further study. The highest production of GABA was obtained within 48h from the de Man, Rogosa and Sharpe (MRS) medium, having a pH range of 5-6. Increasing the monosodium glutamate (MSG) concentration resulted in a higher accumulation of GABA and reached the highest concentration (15.74 g/L) using 4% (w/v) MSG, while high residual MSG was also observed in accordance with concentration increase. On the other hand, the addition of NaCl in the culture medium by up to 7% (w/v) did not suppress GABA production. Preliminary application of strain L10-11 as starter producing GABA was investigated in Thai fermented vegetables (Som-pak). It was found that GABA formation could be observed increasingly following the concentration of MSG added. GABA content in Som-pak was 5 times higher than that of control when inoculating Lb. plantarum L10-11 and adding MSG at 1% (w/v). In addition, sensory evaluation revealed that addition of this starter culture to Som-pak gave overall acceptability slightly higher than that which allowed fermentation to occur spontaneously. This alternative procedure would be successful for improving the nutritional quality of functional fermented food.
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