Xylitol production by a methanol yeast, Candida boidinii No.2201
1991
Vitchuporn Vongsuvanlert (Kasetsart Univ. Kamphaengsaen Campus, Nakhon Pathom (Thailand). Central Laboratory and Greenhouse Complex)
Methanol yeast, Candida boidinii No. 2201, could produce xylitol and also D-xylulose during cultivation on D-xylose medium. These fermentative products were identified by high performance liquid chromatography. A large amount of xylitol was obtained from a D-xylose medium containing ammonium acetate and yeast extract at the initial pH of 7.0. Maximum productivities of xylitol and enzymes concerned with the production were observed after 4-5 day of cultivation. A D-xylose (100 g/l) medium supplemented with 2 % (v/v) methanol gave higher amounts of xylitol (48.5 g/l) and D-xylulose (3.3 g/l). Enzyme activities for D-xylose reduction, D-xylulose reduction, D-xylose isomerization, and D-xylulose phosphorylation, which could be involved in the xylitol production, were measured in cell-free extracts during cultivation and a possible pathway of xylitol production was discussed.
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