Correlation of peroxidase and esterase isozyme patterns to disease resistance of some important disease of sugarcane
1993
Sermsiri Mayteewarakoon
Fifty cultivars of commercial sugarcane were examined for isozyme variability in the correlation to disease resistance. Peroxidase and esterase isozymes were analyzed on polyacrylamide gel electrophoresis. Fourteen groups of peroxidase isozyme pattern and 10 groups of esterase isozyme pattern were detected. Peroxidase bands with the Rf values of 0.092,0.444,0.583 and 0.629 as well as 5 esterase bands with the Rf between 0.609 - 0.718 were common among sugarcane cultivars. The correlation between peroxidase isozyme pattern and disease resistance of sugarcane was observed. Most of disease resistance varieties to smut fungi (Ustilago scitaminea), stem and root rot fungi (Fusarium moniliforme) correlated to the peroxidase band with the Rf of 0.527 whereas this band was absent in susceptible cultivars. The unique bands with the Rf of 0.410 and 0.418 highly correlated to the susceptibility of sugarcane cultivars to ring spot disease caused by the fungus, Leptosphaeria sacchari and smut fungi, respectively. No significant correlation was found between esterase isozyme pattern and disease resistance level of 15 tested sugarcane varieties. The artificial inoculation of smut fungus into the susceptible sugarcane cultivar, Q 83 showed found that the spore concentration of 10 ** (4) - 10 ** (5) chlamydospores/ml. with the incubation period varied from 0 - 48 hrs gave no significant difference on infection percentage and lethal percentage. The quantitative analysis of peroxidase and esterase isozyme pattern of 10 sugarcane cultivars revealed that no significant difference was detected between isozyme patterns of healthy sugarcane and smut diseased sugarcane.
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