Production of peptone from hybrid clarias catfish and from processing waste
1996
Pitchaya Chinauparvat
Ratio of raw material to water and factors affecting autolysis (temperature and time) of hybrid Clarias catfish processing waste were studied. It was found that the appropriate ratio of raw material: water in autolysis was 1:1 at 37 deg C, initial pH 7.0 for 2 hours. The average soluble protein obtained was 3.09 mg/ml. The appropriate conditions for enzyme hydrolysis were also studied using papain, bromelain and pepsin. The suitable enzyme for hydrolysis was papain with optimal hydrolysis conditions of 0.2 percent enzyme at 40 deg C, initial pH 7.0, for 4 hours. The soluble protein content hydrolyzate was 4.14 mg/ml. Headed hybrid Clarias catfish was used as raw material compared to the processing waste, it was found that under the same conditions, papain hydrolysis yielded the hydrolyzate with 6.25 mg/ml soluble protein. Pepton from headed catfish yielded a product with 86.50 percent protein, 0.03 percent fat, 7.54 percent moisture content, 6.64 percent ash and 1.65 percent sodium chloride. Peptone prepared from processing waste contained 85.60 percent protein, 0.09 percent fat, 6.64 percent moisture content, 8.17 percent ash and 2.85 percent sodium chloride. There were 18 amino acids in both peptones which were able to support growth of Bacillus subtilis, Escherichia coli, and Staphylococcus aureus better than commercial peptone. The yield of peptone from headed catfish and from processing waste were 10.40 and 9.90 percent (wt/wt) of raw material, respectively.
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