Temperature and pH profiling in the production of acetic acid from hydrolysed pineapple waste
1993
Vijayakumar, Raghavan
Selection of suitable cellulase-4000 enzyme to hydrolyse the pineapple waste was investigated and the results showed that cellulase at 0.5 % (w/w) was the most efficient in terms of converting cellulose into fermentable sugar giving a final concentration of 46.27 mg/ml in thirteen hours. pH shifting done to select suitable set points resulted in pH profiling; 0-6 hr period at pH 4.2, 6-24 hr period at pH 3.6 and 24-30 hr at pH 3.0. A maximum of 33.72 ml/kg of acetic acid was produced by pH profiling. Temperature shifting done to select set points resulted in temperature profiling; 0-12 hr period at a temperature 37 deg C, a 12-18 hr period at 32 deg C, a 18-24 hr period at 27 deg C and a 24-30 hr period at 32 deg C are considered for temperature profiling. The maximum of 61.17 ml/kg of acetic acid was produced by temperature profiling. Temperature profiling gave a 27.45 ml/kg yield higher than by pH profiling. Hydrolysed and non-hydrolysed pineapple waste fermented for 48 hours at a constant temperature of 32 deg C and constant pH 3.8, yielded 49.33 ml/kg and 32.54 ml/kg acetic acid respectively. The acetic acid yield from hydrolysed waste and temperature profiling gave a yield of 61.17 g/kg and the yield was 87.9 % higher when compared with the non-hydrolysed fermentation of pineapple waste. In all above fermentations 10 % v/v Acetobactor aceti was used as an inoculum.
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