In vitro propagation of bael (Aegle marmelos Corr.)
1997
Supinya Khomkhajorn
Lateral buds of Bael (Aegle marmelos Corr.) were used as the explants to propagate in vitro. Surface sterilization was done in various concentration of clorox. Twice surface sterilization in clorox 10 percent for 10 minutes and followed by clorox 5 percent 5 minutes gave the best bud growth which was 50 percent. The clean culture of lateral buds were placed on semi-solid MS media (Murachige & Skoog, 1962) supplemented with BA (N6-benzyladenine). BA at 2 mg/L could induced the highest shoot number of 7.8 shoots per bud and the average shoot length was 1.2 cm within 60 days. The shoots from lateral bud were placed on MS media supplemented with BA. BA at 1 mg/L could induced the highest shoot number of 14.2 shoot per single shoot and the average shoot length was 1.75 cm within 60 days. Roots were induced in 1/2 MS with IBA (indole-3-butyric acid). IBA at 1.0 mg/L gave the highest number of roots which was 4.2 roots per shoot and the average length was 3.07 cm within 60 days. The plantlets were transplanted to growing media and covered with plastic bags for moisture control. The highest survival rate was 70 percent for 4 weeks under plastic bags. Cotyledons, young petioles and young leaves were induced to callus on MS media supplemented with NAA (alpha-naphthalene acetic acid) in combination with Kn (kinetin). These media were able to induce callus from all kind of explants within 60 days. Only callus from cotyledons could induced to normal shoots and the highest shoot number was at 0.1 mg/L NAA combining with 1 mg/L BA within 60 days. Callus from young petioles and young leaves were not able to develope shoot. Study on zymograme of bael by mean of polyacrylamide gel electrophoresis, the zymograme of bael from in vitro propagation was as similar as the zymograme of bael in the nature from the same mother plant. This study can concluded that in vitro plantlets have the same heridity as the mother plant.
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