QTL mapping and genetic analysis of rice CMS-WA fertility restoration via linkage of DNA markers
1998
Xue-lin, Tan
Rice CMS-WA cytoplasm is extensively used in various national hybrid rice breeding programs, but reports about number and positions of the genes conferring fertility restoration are inconsistent. Using QTL mapping techniques based on a DNA marker linkage map constructed on a backcross population, this study found that the fertility restoration was mainly controlled by two loci on chromosome 10, which had additive effects. The major locus was also identified with bulked line analysis, a method developed in this study for identifying RFLP markers associated with target genes, and identified by bulked segregants of two populations. Although the conclusion of this study was not consistent with popular concepts that the fertility restoration was controlled 6y one or two independent gene, it is supported by data of a trisomic analysis and most of fertility segregating distribution in this and other studies. Based on the linkage between major Rf genes and RFLP markers detected in this and other studies, this study speculates that rice major fertility restring genes for CMS-WA and CMS-BT systems could have some evolutionary relationship.
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This bibliographic record has been provided by Thai National AGRIS Centre, Kasetsart University
